SiRNA Validation Data

siRNA ID:

s14591

Gene ID:

7329

Transcript(s):

NM_003345.4 NM_194259.2 NM_194260.2 NM_194261.2 XM_005255540.2 XM_005255541.4 XM_011522644.2 XM_011522645.1 XM_017023640.1

Validation Results:

1%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00163336_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.