SiRNA Validation Data

siRNA ID:

s16402

Gene ID:

8622

Transcript(s):

NM_001029851.2 NM_001029852.2 NM_001029853.2 NM_001029854.2 NM_003719.3 XM_005248621.4 XM_005248623.4 XM_005248624.4 XM_006714725.3 XM_006714726.3 XM_011543699.2 XM_011543700.2 XM_011543701.2 XM_011543702.2 XM_011543703.2 XM_011543704.2 XM_017010003.1 XM_017010004.1 XM_017010005.1 XM_017010006.1 XM_017010007.1 XM_017010008.1

Validation Results:

13%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00405493_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.