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SiRNA Validation Data

siRNA ID:

s1750

Gene ID:

489

Transcript(s):

NM_005173.3 NM_174953.2 NM_174954.2 NM_174955.2 NM_174956.2 NM_174957.2 NM_174958.2 XM_011523881.2 XM_011523882.2 XM_011523884.2 XM_011523885.1 XM_011523888.2 XM_011523889.1 XM_011523892.2 XM_017024692.1 XM_017024693.1

Validation Results:

10%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00193090_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.