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SiRNA Validation Data

siRNA ID:

s22515

Gene ID:

22858

Transcript(s):

NM_014920.3 NM_016513.4 XM_011514419.1 XM_011514420.2 XM_011514421.1 XM_017010485.1 XM_017010486.1 XM_017010487.1 XM_017010488.1 XM_017010489.1 XM_017010490.1 XM_017010491.1 XM_017010492.1

Validation Results:

18%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00248170_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.