SiRNA Validation Data

siRNA ID:

s25625

Gene ID:

26750

Transcript(s):

NM_001136138.2 NM_001287218.1 NM_001287219.1 NM_001287220.1 NM_001287221.1 NM_012424.4 XM_005273095.1 XM_005273096.1 XM_006711272.1 XM_017001024.1 XM_017001025.1 XM_017001026.1 XM_017001027.1 XM_017001028.1 XM_017001029.1 XM_017001030.1 XM_017001031.1 XM_017001032.1 XM_017001033.1 XM_017001034.1

Validation Results:

8%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00179396_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.