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SiRNA Validation Data

siRNA ID:

s4073

Gene ID:

1716 100874048

Transcript(s):

NM_001318859.1 NM_001318860.1 NM_001318861.1 NM_001318862.1 NM_001318863.1 NM_080916.2 NM_080918.2 NR_134893.1 NR_134894.1 NR_134895.1 NR_134896.1 NR_134897.1 NR_134898.1 XM_011532647.2

Validation Results:

4%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00176514_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.