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SiRNA Validation Data

siRNA ID:

s5350

Gene ID:

2356

Transcript(s):

NM_001018078.2 NM_001288803.1 NM_004957.5 NR_110170.1 XM_005251864.3 XM_011518439.2 XM_017014565.1 XM_017014566.1

Validation Results:

15%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00191956_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.