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SiRNA Validation Data

siRNA ID:

s8931

Gene ID:

4351

Transcript(s):

NM_001289155.1 NM_001289156.1 NM_001289157.1 NM_002435.2 XM_011521592.1 XM_011521593.2 XM_017022207.1 XM_017022208.1 XM_017022209.1

Validation Results:

6%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00159228_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.