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SiRNA Validation Data

siRNA ID:

s14372

Gene ID:

7182

Transcript(s):

NM_001291694.1 NM_003298.4 XM_011534058.2 XM_011534059.2 XM_011534061.2 XM_011534063.2 XM_011534064.2 XM_011534065.2 XM_011534066.2 XM_017007118.1 XM_017007119.1 XM_017007120.1

Validation Results:

16%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00231489_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.