SiRNA Validation Data

siRNA ID:

104675

Gene ID:

Transcript(s):

NM_001177310.1 NM_001177311.1 NM_017662.4 XM_011518244.1 XM_011518245.1 XM_011518246.1 XM_011518247.1 XM_011518248.1 XM_011518250.1 XM_011518251.2 XM_011518252.2 XM_011518255.2 XM_017014289.1

Validation Results:

27%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00214306_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.