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SiRNA Validation Data

siRNA ID:

1424

Gene ID:

4882

Transcript(s):

NM_003995.3 XM_005251478.3 XM_005251479.4 XM_011517890.2 XM_011517891.2 XM_011517892.2 XM_011517893.2 XM_011517895.2 XM_017014745.1 XM_017014746.1 XM_017014747.1 XM_017014748.1 XM_017014749.1 XM_017014750.1

Validation Results:

17%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.