SiRNA Validation Data

siRNA ID:

17522

Gene ID:

Transcript(s):

NM_001104631.1 NM_001165899.1 NM_001197218.1 NM_001197219.1 NM_001197220.1 NM_001197221.1 NM_001197222.1 NM_001197223.1 NM_006203.4 XM_005248537.3 XM_005248538.4 XM_011543469.1 XM_011543470.2 XM_011543471.1 XM_011543473.1 XM_011543477.2 XM_017009565.1 XM_017009566.1 XM_017009567.1 XM_017009568.1 XM_017009569.1 XM_017009570.1 XM_017009571.1

Validation Results:

6%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00174810_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.