SiRNA Validation Data

siRNA ID:

26257

Gene ID:

Transcript(s):

NM_001258315.1 NM_001258316.1 NM_018098.5 XM_005247176.1 XM_006713523.3 XM_011512514.2 XM_011512515.2 XM_011512516.1 XM_017005830.1 XM_017005831.1 XM_017005832.1 XM_017005833.1 XM_017005834.1 XM_017005835.1 XM_017005836.1 XM_017005837.1 XM_017005838.1

Validation Results:

10%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00216455_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.