SiRNA Validation Data

siRNA ID:

37423

Gene ID:

Transcript(s):

NM_001278649.1 NM_014835.3 NM_144498.2 XM_017028163.1 XM_017028164.1 XM_017028165.1 XM_017028166.1 XM_017028167.1 XM_017028168.1 XM_017028169.1 XM_017028170.1

Validation Results:

11%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00375886_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.