19024144
NM_001401.3 NM_057159.2 XM_005251781.3 XM_005251782.3 XM_017014383.1 XM_017014384.1 XM_017014385.1 XM_017014386.1 XM_017014387.1 XM_017014388.1 XM_017014389.1 XM_017014390.1 XM_017014391.1 XM_017014392.1 XM_017014393.1 XM_017014394.1 XM_017014395.1 XM_017014396.1 XM_017014397.1 XM_017014398.1 XM_017014399.1 XM_017014400.1 XM_017014401.1 XM_017014402.1 XM_017014403.1 XM_017014404.1 XM_017014405.1 XM_017014406.1 XM_017014407.1
26%(+/-10% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00173500_m1]
HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.