5747707
NM_001199649.1 NM_005607.4 NM_153831.3 XM_005251003.2 XM_005251012.2 XM_006716608.2 XM_011517194.2 XM_017013651.1 XM_017013652.1 XM_017013653.1 XM_017013654.1 XM_017013655.1 XM_017013656.1 XM_017013657.1 XM_017013658.1 XM_017013659.1 XM_017013660.1 XM_017013661.1 XM_017013662.1 XM_017013663.1 XM_017013664.1 XM_017013665.1 XM_017013666.1 XM_017013667.1 XM_017013668.1 XM_017013669.1 XM_017013670.1 XM_017013671.1 XM_017013672.1 XM_017013673.1 XM_017013674.1 XM_017013675.1 XM_017013676.1 XM_017013677.1 XM_017013678.1 XM_017013679.1 XM_017013680.1
19%(+/-0% mRNA remaining)(HeLa cell line)
The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.