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SiRNA Validation Data

siRNA ID:

s10372

Gene ID:

5214

Transcript(s):

NM_001242339.1 NM_001323067.1 NM_001323068.1 NM_001323069.1 NM_001323070.1 NM_001323071.1 NM_001323072.1 NM_001323073.1 NM_001323074.1 NM_002627.4 XM_005252465.4 XM_005252466.4 XM_005252467.2 XM_006717449.1

Validation Results:

9%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00737354_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.