SiRNA Validation Data

siRNA ID:

s148

Gene ID:

2889

Transcript(s):

NM_001304275.1 NM_005312.3 NM_198679.1 XM_005272186.4 XM_005272191.3 XM_006717067.3 XM_006717072.3 XM_006717074.3 XM_011518569.2 XM_011518570.2 XM_011518571.2 XM_011518572.2 XM_011518573.2 XM_011518574.2 XM_011518575.2 XM_011518576.2 XM_011518577.2 XM_011518578.2 XM_011518579.2 XM_011518580.2 XM_011518581.2 XM_011518582.2 XM_017014633.1 XM_017014634.1 XM_017014635.1 XM_017014636.1 XM_017014637.1 XM_017014638.1 XM_017014639.1 XM_017014640.1 XM_017014641.1

Validation Results:

10%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00178409_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.