SiRNA Validation Data

siRNA ID:

s224168

Gene ID:

5066

Transcript(s):

NM_000919.3 NM_001177306.1 NM_001319943.1 NM_138766.2 NM_138821.2 NM_138822.2 NR_033440.1 XM_011543419.2 XM_017009495.1 XM_017009496.1 XM_017009497.1 XM_017009498.1 XM_017009499.1 XM_017009500.1 XM_017009501.1 XM_017009502.1 XM_017009503.1 XM_017009504.1 XM_017009505.1 XM_017009506.1 XM_017009507.1 XM_017009508.1 XM_017009509.1 XM_017009510.1 XM_017009511.1 XM_017009512.1 XM_017009513.1 XM_017009514.1 XM_017009515.1 XM_017009516.1

Validation Results:

9%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00168596_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.