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SiRNA Validation Data

siRNA ID:

s29516

Gene ID:

54822

Transcript(s):

NM_001301212.1 NM_017672.5 XM_005254486.3 XM_017022350.1 XM_017022351.1 XM_017022352.1 XM_017022353.1 XM_017022354.1 XM_017022355.1 XM_017022356.1

Validation Results:

20%(+/-4% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00292383_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.