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SiRNA Validation Data

siRNA ID:

s5007

Gene ID:

2185

Transcript(s):

NM_004103.4 NM_173174.2 NM_173175.2 NM_173176.2 XM_005273447.4 XM_005273448.4 XM_011544441.2 XM_011544442.2 XM_017013214.1 XM_017013215.1 XM_017013216.1

Validation Results:

14%(+/-10% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00169444_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.