SiRNA Validation Data

siRNA ID:

s8355

Gene ID:

4067

Transcript(s):

NM_001111097.2 NM_002350.3 XM_011517529.2 XM_017013415.1 XM_017013416.1

Validation Results:

18%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00176719_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.