SiRNA Validation Data

siRNA ID:

114375

Gene ID:

8829

Transcript(s):

NM_001024628.2 NM_001024629.2 NM_001244972.1 NM_001244973.1 NM_003873.5 NR_045259.1 XM_006717521.1 XM_006717522.1 XM_006717523.2 XM_006717524.1 XM_006717525.1 XM_006717526.1 XM_011519755.2 XM_011519756.1 XM_017016865.1 XM_017016866.1

Validation Results:

7%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00826129_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.