SiRNA Validation Data

siRNA ID:

117312

Gene ID:

Transcript(s):

NM_005116.5 NM_203327.1 XM_011529414.2 XM_011529415.2 XM_011529416.2 XM_011529417.2 XM_017028171.1 XM_017028172.1 XM_017028173.1 XM_017028174.1 XM_017028176.1 XM_017028177.1 XM_017028178.1 XM_017028179.1 XM_017028181.1

Validation Results:

12%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00192765_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.