SiRNA Validation Data

siRNA ID:

4014

Gene ID:

7068

Transcript(s):

NM_000461.4 NM_001128176.2 NM_001128177.1 NM_001252634.1 XM_005265421.4 XM_005265423.4 XM_005265424.3 XM_006713317.3 XM_006713318.3 XM_011534046.2 XM_011534047.2 XM_011534048.2 XM_011534049.2 XM_011534050.2 XM_011534051.2 XM_011534052.2 XM_011534053.2 XM_017007108.1 XM_017007109.1 XM_017007110.1 XM_017007111.1 XM_017007112.1 XM_017007113.1 XM_017007114.1 XM_017007115.1

Validation Results:

26%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.