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SiRNA Validation Data

siRNA ID:

490

Gene ID:

5576

Transcript(s):

NM_001321982.1 NM_001321983.1 NM_001321989.1 NM_004157.3 XM_005265315.3 XM_011533942.2 XM_011533943.1 XM_017006815.1 XM_017006816.1

Validation Results:

9%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00177760_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.