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SiRNA Validation Data

siRNA ID:

s10363

Gene ID:

5211

Transcript(s):

NM_001002021.2 NM_002626.5 XM_005261135.3 XM_005261137.4 XM_006724011.1 XM_006724012.1 XM_011529603.2 XM_017028368.1 XM_017028369.1 XM_017028370.1 XM_017028371.1 XM_017028372.1

Validation Results:

9%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00160027_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.