Close X

SiRNA Validation Data

siRNA ID:

s10971

Gene ID:

5521

Transcript(s):

NM_001271899.1 NM_001271900.1 NM_001271948.1 NM_181674.2 NM_181675.3 NM_181676.2 NM_181677.2 NM_181678.2 NR_073526.1 NR_073527.1

Validation Results:

15%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00270227_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.