SiRNA Validation Data

siRNA ID:

s16319

Gene ID:

8569

Transcript(s):

NM_001135553.2 NM_003684.5 NM_198973.3 NR_024174.2 NR_024176.2 XM_006711000.1 XM_006711001.2 XM_006711002.2 XM_006711003.1 XM_006711006.3 XM_006711007.1 XM_006711009.3 XM_006711013.1 XM_006711014.1 XM_006711015.1 XM_017002652.1 XM_017002653.1 XM_017002654.1 XM_017002655.1 XM_017002656.1 XM_017002657.1 XM_017002658.1 XM_017002659.1 XM_017002660.1 XM_017002661.1 XM_017002662.1 XM_017002663.1 XM_017002664.1 XM_017002665.1 XM_017002666.1 XM_017002667.1 XM_017002668.1

Validation Results:

15%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00374376_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.