SiRNA Validation Data

siRNA ID:

s20833

Gene ID:

10611

Transcript(s):

NM_001011513.3 NM_001011515.2 NM_001011516.2 NM_001256425.1 NM_001256426.1 NM_001256427.1 NM_006457.4 XM_005262693.4 XM_005262695.4 XM_005262696.4 XM_005262698.4 XM_006714066.3 XM_006714068.3 XM_006714069.3 XM_006714070.3 XM_011531543.2 XM_017007657.1 XM_017007658.1

Validation Results:

18%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00179051_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.