SiRNA Validation Data

siRNA ID:

s2366

Gene ID:

Transcript(s):

NM_001221.3 NM_001321566.1 NM_001321567.1 NM_001321568.1 NM_001321569.1 NM_001321570.1 NM_001321571.1 NM_001321572.1 NM_001321573.1 NM_001321574.1 NM_001321575.1 NM_001321576.1 NM_001321577.1 NM_001321578.1 NM_001321579.1 NM_001321580.1 NM_001321581.1 NM_001321582.1 NM_001321583.1 NM_001321584.1 NM_001321585.1 NM_001321586.1 NM_001321587.1 NM_001321588.1 NM_001321589.1 NM_001321590.1 NM_001321591.1 NM_172114.1 NM_172115.2 NM_172127.2 NM_172128.2 NM_172129.1 XM_005263253.3 XM_005263254.3 XM_006714330.2 XM_011532289.1 XM_011532290.1 XM_011532291.1 XM_011532292.2 XM_011532295.1 XM_017008672.1 XM_017008673.1 XM_017008674.1 XM_017008675.1 XM_017008676.1 XM_017008677.1 XM_017008678.1

Validation Results:

14%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00241833_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.