SiRNA Validation Data

siRNA ID:

s276

Gene ID:

112398 100529264

Transcript(s):

NM_053046.3 NM_080732.3 NR_037791.1

Validation Results:

16%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00363196_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer® Select siRNA or 5 nM Silencer® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.