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SiRNA Validation Data

siRNA ID:

s382

Gene ID:

51699

Transcript(s):

NM_001282150.1 NM_001282151.1 NM_016226.4 NM_057180.2 NR_104099.1 NR_104100.1 NR_104101.1 NR_104102.1 XM_006719459.3 XM_006719460.3 XM_011538484.2 XM_017019457.1 XM_017019458.1 XM_017019459.1

Validation Results:

12%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00372957_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.