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SiRNA Validation Data

siRNA ID:

5348

Gene ID:

5465

Transcript(s):

NM_001001928.2 NM_005036.4 XM_005261655.3 XM_005261656.3 XM_006724269.3 XM_006724270.3 XM_011530239.2 XM_011530240.2 XM_011530241.2 XM_011530242.2 XM_011530243.2 XM_011530244.2 XM_011530245.2 XM_017028839.1 XM_017028840.1

Validation Results:

9%(+/-0% mRNA remaining)(HeLa cell line)

Validation Procedure:

The above cell line was plated in at least triplicate the day before the transfection procedure was started. siRNA was transfected at a final concentration of 100 nM. RNA was extracted from the cells 48 hours after transfection. mRNA levels were measured by real-time RT-PCR using a random primer mix for the RT priming, followed by real time PCR. Knockdown is expressed as the relative amount of target mRNA remaining compared to that of cells transfected with a "nonsense" or scrambled siRNA negative control. Validation results are shown for multiple independent experiments.