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SiRNA Validation Data

siRNA ID:

s8223

Gene ID:

4000

Transcript(s):

NM_001257374.2 NM_001282624.1 NM_001282625.1 NM_001282626.1 NM_005572.3 NM_170707.3 NM_170708.3 XM_011509533.1 XM_011509534.1

Validation Results:

5%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00153462_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.