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SiRNA Validation Data

siRNA ID:

114050

Gene ID:

5728

Transcript(s):

NM_000314.6 NM_001304717.2 NM_001304718.1

Validation Results:

23%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00829813_s1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.