SiRNA Validation Data

siRNA ID:

118472

Gene ID:

Transcript(s):

NM_001173463.1 NM_001173464.1 NM_001173465.1 NM_017641.3 XM_005269007.2 XM_005269008.2 XM_005269009.2 XM_005269010.2 XM_005269011.2 XM_005269012.2 XM_005269013.2 XM_005269014.2 XM_006719493.2 XM_006719494.2 XM_011538556.2 XM_017019607.1 XM_017019608.1 XM_017019609.1 XM_017019610.1 XM_017019611.1

Validation Results:

8%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00286908_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.