SiRNA Validation Data

siRNA ID:

119910

Gene ID:

9351

Transcript(s):

NM_001130012.2 NM_001252073.1 NM_001252075.1 NM_001252076.1 NM_004785.5 XM_005255702.4 XM_006720981.1 XM_017023894.1 XM_017023895.1

Validation Results:

2%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00191186_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.