SiRNA Validation Data

siRNA ID:

s10026

Gene ID:

5063

Transcript(s):

NM_001128166.2 NM_001128167.2 NM_001128168.2 NM_001128172.2 NM_001128173.2 NM_001324325.1 NM_001324326.1 NM_001324327.1 NM_001324328.1 NM_001324329.1 NM_001324330.1 NM_001324331.1 NM_001324332.1 NM_001324333.1 NM_001324334.1 NM_002578.4 NR_136747.1 NR_136748.1 XM_005262132.2 XM_005262133.3 XM_006724654.2 XM_006724655.2 XM_011530962.1 XM_011530967.2 XM_011530968.2 XM_011530969.1 XM_017029557.1 XM_017029558.1 XM_017029559.1 XM_017029560.1 XM_017029561.1 XM_017029562.1 XM_017029563.1

Validation Results:

7%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00176828_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.