SiRNA Validation Data

siRNA ID:

s16583

Gene ID:

8706

Transcript(s):

NM_001038628.1 NM_003781.3 NM_033167.2 NM_033168.2 NM_033169.2 XM_005247859.4 XM_005247861.3 XM_005247862.3 XM_005247864.3 XM_006713803.3 XM_006713804.3 XM_006713805.3 XM_017007402.1 XM_017007403.1 XM_017007404.1 XM_017007405.1 XM_017007406.1 XM_017007407.1 XM_017007408.1 XM_017007409.1 XM_017007410.1 XM_017007411.1 XM_017007412.1

Validation Results:

17%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00364202_s1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.