SiRNA Validation Data

siRNA ID:

s16718

Gene ID:

8776

Transcript(s):

NM_001306144.1 NM_001306145.1 NM_003828.3 XM_005274765.3 XM_005274766.3 XM_005274767.3 XM_006724855.3 XM_011531209.2 XM_011531210.2 XM_017029920.1 XM_017029921.1 XM_017029922.1 XM_017029923.1 XM_017029924.1

Validation Results:

13%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00382245_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.