SiRNA Validation Data

siRNA ID:

s26897

Gene ID:

30011

Transcript(s):

NM_001024666.2 NM_001184960.1 NM_031892.2 XM_005274494.2 XM_011545498.2 XM_011545499.2 XM_011545500.2 XM_011545502.2 XM_017029459.1 XM_017029460.1 XM_017029461.1 XM_017029462.1 XM_017029463.1 XM_017029464.1 XM_017029465.1 XM_017029466.1 XM_017029467.1 XM_017029468.1 XM_017029469.1 XM_017029470.1

Validation Results:

6%(+/-0% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00230414_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.