SiRNA Validation Data

siRNA ID:

s4692

Gene ID:

2030

Transcript(s):

NM_001078175.2 NM_001078177.1 NM_001304462.1 NM_001304463.1 NM_001304465.1 NM_001304466.1 XM_005248876.4 XM_005248878.3 XM_005248879.3 XM_005248880.3 XM_005248881.3 XM_005248882.3 XM_011514341.2

Validation Results:

14%(+/-5% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00191940_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer® Select siRNA or 5nM Silencer® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan® Gene Expression Cells-to-CT™ Kit and appropriate TaqMan® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.