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SiRNA Validation Data

siRNA ID:

s556

Gene ID:

10277

Transcript(s):

NM_001105562.2 NM_006048.4 XM_005263422.1 XM_011540488.2 XM_011540489.2 XM_011540490.1 XM_017000035.1 XM_017000036.1

Validation Results:

10%(+/-3% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00195897_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.