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SiRNA Validation Data

siRNA ID:

s7403

Gene ID:

3609

Transcript(s):

NM_001137673.1 NM_004516.3 NM_012218.3 NM_017620.2 NM_153464.2 XM_006722742.2 XM_011527984.2 XM_011527985.2 XM_011527987.1 XM_017026763.1 XM_017026764.1 XM_017026765.1

Validation Results:

8%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs01128111_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5 nM gene-specific Silencer^® Select siRNA or 5 nM Silencer^® Select Negative Control #2 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step, quantitative RT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.