Close X

SiRNA Validation Data

siRNA ID:

s9997

Gene ID:

5048

Transcript(s):

NM_000430.3 XM_011523901.1 XM_011523902.2 XM_011523903.2 XM_017024699.1 XM_017024700.1 XM_017024701.1 XM_017024702.1

Validation Results:

20%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00181182_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.