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SiRNA Validation Data

siRNA ID:

139260

Gene ID:

8517

Transcript(s):

NM_001099856.4 NM_001099857.2 NM_001145255.2 NM_001321396.1 NM_001321397.1 NM_003639.4

Validation Results:

8%(+/-1% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00415849_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.