Close X

SiRNA Validation Data

siRNA ID:

s7727

Gene ID:

3755

Transcript(s):

NM_002237.3 XM_006723785.2 XM_011528800.1 XM_011528801.1 XM_011528802.1 XM_011528803.2 XM_011528804.1 XM_011528805.2 XM_011528806.1

Validation Results:

9%(+/-2% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00158410_m1]

Validation Procedure:

HeLa cells were reverse transfected with 5nM gene-specific Silencer^® Select siRNA or 5nM Silencer^® Select Negative Control #2 siRNA. 48 hours post-transfection, gene expression analysis was performed using the TaqMan^® Gene Expression Cells-to-CT^™ Kit and appropriate TaqMan^® Gene Expression Assays. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.