SiRNA Validation Data



Gene ID:



NM_001135954.2 NM_001261450.2 NM_001261451.2 NM_001318519.1 NM_031432.3 NR_134666.1 NR_134667.1 XM_005272224.2

Validation Results:

20%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan® Assay ID: Hs00258815_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer® Negative Control #1 siRNA, using siPORT™ NeoFX™ Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.