SiRNA Validation Data

siRNA ID:

111403

Gene ID:

Transcript(s):

NM_001282112.1 NM_001282113.1 NM_003935.4 XM_005261811.1 XM_005261813.2 XM_006724349.1 XM_006724350.1 XM_011530482.1 XM_017029038.1 XM_017029039.1 XM_017029040.1 XM_017029041.1

Validation Results:

27%(+/-7% mRNA remaining)(HeLa cell line) [TaqMan^® Assay ID: Hs00172728_m1]

Validation Procedure:

HeLa cells were reverse transfected with 30 nM gene-specific siRNA or 30 nM Silencer^® Negative Control #1 siRNA, using siPORT NeoFX Transfection Agent. 48 hours post-transfection, RNA was isolated, and then two-step qRT-PCR was performed using an appropriate TaqMan^® Gene Expression Assay. 18S rRNA was used as an endogenous control. Validation results are expressed as average percent mRNA remaining, relative to Negative Control siRNA-treated samples, along with the standard deviation of multiple independent experiments.